Hiway 17 north from Deland turns into a nice 2 lane road. Hiway 19 north of Eustis is a good road. Takes you into the Ocala Forest. Hiway 44 east of Deland and hiway 42 are both good. If you go across 42 towards Weirsdale head north on 25 to Ma Barkers and Gator Joes.
314 is another nice road in the Forest. Anytime you ride through the Oacla Forest, keep an eye out for bears running in front of you. This has happened to my wife and I.
Heading to Key West, we like to take 441 south from Hiway 192. Takes you around Lake Okeechobee. You can pick up Hiway 27 go south to Krome Ave (sp?). Watch your gas not to many places to stop last time I was through.